Proteomic and immunoassay characterization of a new food allergen from hazelnut (corylus avellana)
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چکیده
منابع مشابه
Proteomic and immunoassay characterization of a new food allergen from hazelnut (corylus avellana)
Methods Otherwise healthy children with oral food challenge confirmed hazelnut allergy were prospectively evaluated. Crude protein extracts were obtained from 5 hazelnut varieties, including autochthon, Northern Italy and Oregon (USA) cultivars, with phosphate saline buffer, pH 7.2. The immunoreactive protein components were identified by SDS-PAGE electrophoresis and Western immunoblotting, usi...
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متن کاملHigh Quality RNA Isolation from Leaf, Shell, Root Tissues and Callus of Hazelnut (Corylus avellana L.)
Extraction of high quality RNA is a critical step in molecular genetics studies. Hazelnut is one of the most important nuts plants in the world. The presence of the taxol and other taxanes in hazelnut plant necessitates explaining their biosynthesis pathway and identifying the candidate genes. Therefore, an easy and practical method is necessary for RNA extraction from hazelnuts. Hazelnut has h...
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ژورنال
عنوان ژورنال: Clinical and Translational Allergy
سال: 2013
ISSN: 2045-7022
DOI: 10.1186/2045-7022-3-s3-p40